AMIR RMAILE THESIS

In this thesis, fundamental studies to characterize the water content, swelling by different salts, annealing effect, viscoelastic and mechanical properties of polyelectrolyte multilayers and complexes are illustrated and conducted. Ion-pairing and water content are key factors influencing permeability of molecules into polyelectrolyte multilayers. The results detailed the degradation effect of each enzyme, separately and in combination. Various methods to measure mechanical properties of dental biofilms were investigated, and physical and chemical strategies to disrupt these biofilms were employed. Furthermore, the influence of fluid shear flow on the detachment of Streptococcus mutans biofilms inside microfluidic channels was studied using a commercially available flow-cell system.

In this thesis, fundamental studies to characterize the water content, swelling by different salts, annealing effect, viscoelastic and mechanical properties of polyelectrolyte multilayers and complexes are illustrated and conducted. The elastic modulus increased by increasing the sucrose percentage in the media, and decreased when the biofilms were treated with increasing concentrations of ethylene di-amine tetra acetic acid, EDTA. Treating the biofilms with different solutions of poly ethylene glycol , PEG, resulted in behaviour similar to that previously observed for synthetic polymers. Other digital versions may also be available to download e. The film loses extrinsic sites as it anneals. They are also important in controlling the multilayer mechanical properties which are especially important when considering the use of PEMUs in biomaterials applications. The enzymes used were:

Furthermore, pre-coating amir rmaile thesis plates with the three enzymes did not inhibit biofilm from formation and accumulation. Abstract A literature review of dental plaque biofilms formation, progression and detachment mechanisms is presented in this thesis. The multilayer resembles a polymeric network comprising regions of varying intensities of polyion-polyion interactions the extent amir rmaile thesis distribution of which are sensitive to the type of the polyelectrolyte used and to the type and concentration of the salt in the surrounding medium.

A critical biofilm detachment shear stress was estimated for the large biofilm-aggregates CDSSagg. A uniaxial compressive test was utilized to characterize the mechanical behaviour of biofilms, while manipulating the chemical microenvironment. Amir rmaile thesis of Southampton, Faculty of Engineering and the Environment.

Mechanical properties and disruption of dental biofilms. The enzymes used were: Skip to main content. A self assembled multilayer technique based on alternating deposition of oppositely charged polyelectrolytes onto charged solid substrates is described in this thesis.

High-speed imaging, was used to characterise the PT-AirFloss microburst of pressurized air and water micodrops, and demonstrated the removal mechanism of a dental plaque biofilm substitute and the S. High velocity water microdrops, with minimal fluid volume and time, effectively removed both the surrogate and the biofilm.

It rather creates enough osmotic pressure to pull water out from the Amir rmaile thesis. Furthermore, the influence of fluid shear flow on the detachment of Streptococcus mutans biofilms inside microfluidic channels was studied using a commercially available flow-cell system.

Full Description View Document Title: The flow field and local hydrodynamics of high velocity water microdrops impacting the interproximal IP space of typodont teeth, and their influence on the structure and detachment of both surrogate dental plaque and Amir rmaile thesis mutans biofilms, were studied experimentally and computationally. Their water content, mechanical properties, thermal properties, and swelling behavior all encourage us to apply them in the biomedical field, in particular as bio-implants amir rmaile thesis replace intervertebral disc in the spinal cord.

Mechanical properties and disruption of dental biofilms – ePrints Soton

In this thesis, fundamental studies to characterize the water content, swelling by different salts, annealing amir rmaile thesis, viscoelastic and mechanical properties of polyelectrolyte multilayers and complexes are illustrated and conducted. The shear stress distribution generated by the drop impacting the tooth surface was calculated by Computational Fluid Dynamics CFD simulations based on the finite element method FEM. Characterization of Polyelectrolyte Multilayers and Complexes: The elastic modulus amir rmaile thesis by increasing the sucrose percentage in the media, and decreased when the biofilms were treated with increasing concentrations of ethylene di-amine tetra acetic acid, EDTA.

Characterization of Polyelectrolyte Multilayers and Complexes. Also, there was a large variability which could be explained by the heterogeneity of the biofilm. Osmotic pressure experiment is a good approach to characterize the water content inside a PEMU. Library staff amir rmaile thesis information.

Dynamic mechanical analysis DMAthermogravimetric analysis TGAand mechanical stretching machines were used to conduct different experiments on those complexes. The focus of the thesis was on the mechanical properties and disruption of dental biofilms, especially from hard-to-access areas of the oral cavity, such as the interproximal IP sites between the teeth.

Water droplets of ? The three enzymes demonstrated different efficacies in degrading the biofilm in 6, 24 and 96 well-plates, as well amir rmaile thesis on the typodont teeth. If you continue without changing your amir rmaile thesis, we will assume that you are happy to receive cookies on the University of Southampton website. Amir rmaile thesis are still investigating the different properties of those complexes and working on enhancing their structure and biocompatibility.

Characterization of Polyelectrolyte Multilayers and Complexes |

After optimizing the amir rmaile thesis with the gel, the mechanical properties of S. Biochemistry Biophysics Molecular biology Chemistry. Next the effect of three biofilm matrix-degrading enzymes on the structure and detachment of Streptococcus mutans biofilms inside microtiter plates and on typodont teeth was studied experimentally.

We were able to get preliminary data about amir rmaile thesis the elastic modulus and the shear modulus of those complexes. Bromelain a proteaseDNase, and RNase. Download statistics Downloads from ePrints over the past year.

Polyethylene glycol, PEG, a highly water-soluble polymer, was used as an osmotic amir rmaile thesis agent. Various strategies that have been employed to reduce or eliminate dental biofilms are discussed. Various methods to measure mechanical properties of dental biofilms were investigated, and physical and chemical strategies to disrupt these biofilms were employed.

Mechanical properties and disruption of dental biofilms

The main principles and methodologies of this technique are explained in details in amir rmaile thesis effort to develop amir rmaile thesis technologies that would amir rmaile thesis beneficial for making new products or enhancing the quality of existing ones.

In addition, CFD models were used to predict optimal water drop or burst design with respect to more effective biofilm removal performance. Ion-pairing and water content are key factors influencing permeability of molecules into polyelectrolyte multilayers.

Lastly the use of a biocompatible copolymer of methylvinyl ether and maleic anhydride, with excellent mucosal adhesive properties and biocompatibility to improve enzymatic digestion by offering a prolonged contact of the enzymes with the teeth and oral tissues, was investigated.

University of Southampton Institutional Repository. A literature review of dental plaque biofilms formation, progression and detachment mechanisms is presented in this thesis.