The elastic modulus increased by increasing the sucrose percentage in the media, and decreased when the biofilms were treated with increasing concentrations of ethylene di-amine tetra acetic acid, EDTA. Layer by layer buildup of polyelectrolyte multilayers on a germanium crystal and ATR-FTIR measurements of ratios of water to sulfonate and azide to sulfonate peaks allowed us to monitor the annealing of PDADMA-PSS multilayers of different thicknesses in different ionic strength solutions. Water droplets of ? Rmaile, Amir Mechanical properties and disruption of dental biofilms. Next the effect of three biofilm matrix-degrading enzymes on the structure and detachment of Streptococcus mutans biofilms inside microtiter plates and on typodont teeth was studied experimentally. The enzymes used were:
We are still investigating the different properties of those complexes and working on enhancing their structure and biocompatibility. Full Description View Document Title: Rmaile, Amir Mechanical properties and disruption of dental biofilms. A distinction is afforded in terms of the swelling and doping abilities of 14 different salts based on their hydration state. Various strategies that have been employed to reduce or eliminate dental biofilms are discussed. The film loses extrinsic sites as it anneals. Streptococcus mutans, the bacterium responsible for initiation of dental plaque biofilms, was used in our studies.
After optimizing the technique with the amir rmaile thesis, the mechanical properties of S. Next the effect of three biofilm matrix-degrading enzymes on the structure and detachment of Streptococcus mutans biofilms inside microtiter plates and on typodont teeth was studied experimentally.
Initially, the mechanical properties of a dextran gel were characterized. Abstract A literature review of dental plaque biofilms amir rmaile thesis, progression and detachment mechanisms is presented in this thesis. The gel was used as an artificial dental plaque biofilm Chapter 5.
Amir rmaile thesis to main content. Using epifluorescence microscopy and image analysis, quantitative measurements of the percentage surface area coverage were obtained, and the preliminary amir rmaile thesis were consistent with the datafrom the plate reader. University of Southampton, Faculty of Engineering and the Environment. The three enzymes demonstrated different efficacies in degrading the biofilm in 6, 24 and 96 well-plates, as well as on the typodont teeth.
High velocity water microdrops, with minimal fluid volume and time, effectively removed both the surrogate and the biofilm. Various amir rmaile thesis to measure mechanical properties of dental biofilms were investigated, and physical and chemical strategies to disrupt these biofilms were employed. Characterization of Polyelectrolyte Multilayers and Complexes: In this thesis, fundamental studies to characterize the water content, swelling by different salts, annealing amir rmaile thesis, viscoelastic and mechanical properties of polyelectrolyte multilayers and complexes are illustrated and conducted.
High-speed imaging, was used to characterise the PT-AirFloss microburst of pressurized air and water micodrops, and demonstrated the removal mechanism of a dental plaque biofilm substitute and the S. University amir rmaile thesis Southampton Institutional Repository. Biochemistry Biophysics Molecular biology Chemistry.
Mechanical properties and disruption of dental biofilms
The flow field and local hydrodynamics of high velocity water microdrops impacting the interproximal IP space of typodont teeth, and their influence on the structure and detachment of both surrogate dental plaque and Streptococcus mutans biofilms, were studied experimentally and computationally.
The shear stress distribution generated by the drop impacting the tooth surface was calculated by Computational Fluid Dynamics CFD simulations based on the finite element method FEM.
Osmotic pressure experiment is a good approach to characterize the water content inside a PEMU. A critical biofilm detachment shear stress was estimated for the large biofilm-aggregates CDSSagg. Ion-pairing and amir rmaile thesis content are key factors influencing permeability of molecules into polyelectrolyte multilayers. Using various microscopy and image analysis techniques, quantitative measurements of the removal rate and the percentage removal of biofilms from different locations in the IP space were obtained.
Also, there was a large variability which could be explained by the heterogeneity amir rmaile thesis the biofilm. The main principles and methodologies of this technique are explained in details in an effort to develop new technologies that would be beneficial for making new products or enhancing the quality of existing ones.
Treating amir rmaile thesis biofilms with different solutions of amir rmaile thesis ethylene glycolPEG, resulted in behaviour similar to that previously observed for synthetic polymers.
A distinction is afforded in terms of the swelling and doping abilities of 14 different salts based on their hydration state. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.
Mechanical properties and disruption of dental biofilms – ePrints Soton
Layer by layer buildup of polyelectrolyte multilayers on a germanium crystal and ATR-FTIR measurements of ratios of water to sulfonate and azide to sulfonate peaks allowed us to monitor the annealing of PDADMA-PSS multilayers of different thicknesses in different ionic strength solutions.
Water droplets of ? amir rmaile thesis
Characterization of Polyelectrolyte Multilayers and Complexes. Furthermore, pre-coating the plates with the three enzymes did not amir rmaile thesis biofilm from formation and accumulation. More information Published date: In addition, CFD models were used to predict optimal water drop or burst design with respect to more effective biofilm removal performance. The focus of the thesis was on the mechanical properties and disruption of dental biofilms, amir rmaile thesis from hard-to-access areas of the oral cavity, such as the interproximal IP sites between the teeth.
Various strategies that have been employed to reduce or eliminate dental biofilms are discussed. The film loses extrinsic sites as it anneals.
A literature review of dental plaque biofilms formation, progression and detachment mechanisms is presented in this thesis. We are still investigating the different properties of those complexes and working on enhancing their structure and biocompatibility. The rationale was amir rmaile thesis eliminate a major obstacle facing the efficacy of the enzymatic therapy which was the relatively short residence time of the enzymes at the amir rmaile thesis of administration.
A good estimate of the number of water molecules inside a multilayer was achieved using PEG. The results detailed the degradation effect of each enzyme, separately and in combination. It rather creates enough osmotic pressure to pull water out from the PEMU.
The adhesive copolymer could possibly enhance enzyme biofilm degradation. A uniaxial compressive test was utilized to characterize the mechanical behaviour of biofilms, while manipulating the chemical microenvironment. The multilayer resembles a polymeric network comprising regions of varying intensities of amir rmaile thesis interactions the extent and distribution of which are sensitive to the type of the polyelectrolyte used and to the type and concentration of the salt in the surrounding medium.
There was good agreement between experimentally measured biofilm removal and the pattern of predicted wall shear stress? Rmaile, Amir Mechanical properties and disruption of dental biofilms.
Mechanical properties and disruption of dental biofilms.
The synthesis of those two polyzwetterions PZs is amir rmaile thesis of many steps taken towards producing biocompatible complexes that can be used as bio-implants. Combining the adhesive copolymer with the enzymes could potentially allow near total degradation of the laboratory-grown S. Download statistics Downloads from ePrints over the amir rmaile thesis year.
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